ECL PRIME WESTERN BLOTTING DETECTION REA

Analysis Note

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Application

ECL^™ Prime Western blotting detection reagent has been used to detect chemiluminescent signals during Western blotting.

Components

The following components are included in the Amersham ECL Prime detection reagent kit.• RPN2236 Solution A: Luminol solution, 3 × 50 ml Solution B: Peroxide solution, 3 × 50 ml sufficient for 3000 cm² membrane

Features and Benefits

• Compatible with Rainbow Molecular Weight Markers and Amersham^™ ECL DualVue^™ Western Blotting Markers.• High signal intensity and sensitivity allows the use of highly diluted primary and secondary antibodies with no reduction in sensitivity.• Stable signal allows multiple exposures and makes the reagent suitable for large experimental series, allowing convenient handling time between the end of the experiment and detection.• Optimized for use with Amersham Hybond-P^® (PVDF) membranes and compatible with Amersham Hybond-ECL (nitrocellulose) membranes.• Optimized for imaging with ImageQuant LAS series (CCD-based imaging) and compatible with Amersham Hyperfilm^™.

General description

Amersham ECL Prime is a highly sensitive reagent, characterized by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. The high intensity of the emitted signals means that proteins can be detected using highly diluted primary and secondary antibodies, contributing to low background and reducing consumption of reagents.Amersham ECL Prime consolidates and builds on the benefits of Amersham ECL Plus and Amersham ECL Advance to deliver a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents.

Legal Information

ECL DualView is a trademark of Amersham

Hyperfilm is a trademark of GE Healthcare

Hybond is a registered trademark of GE Healthcare Companies

ECL is a trademark of GE Healthcare

Amersham is a trademark of GE Healthcare